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US 11,697,797 B2Granted
Manufacturing method of a cell structure
AssigneeNational Cerebral and Cardiovascular Center
InventorsYasuhide Nakayama, Ryosuke Iwai, Yasushi Nemoto
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Abstract
The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises producing a coated region in which a culturing surface is coated with a temperature-responsive polymer or a temperature-responsive polymer composition, forming a droplet of a cell suspension in the coated region, and performing cell culturing in the droplet. A surface zeta potential of the coated region is 0 mV to 50 mV.
Claims8
A manufacturing method of a cell structure, the manufacturing method comprising:
- producing a coated region in which a culturing surface is coated with a temperature-responsive polymer or a temperature-responsive polymer composition, forming a droplet of a cell suspension in the coated region, and performing cell culturing in the droplet;
- wherein a surface zeta potential of the coated region is 0 mV to 50 mV.
Dependencies are inferred from the claim text.