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US 11,697,796 B2Granted
Media and methods for enhancing the survival and proliferation of stem cells
AssigneeStemcell Technologies Canada Inc.
InventorsAdam Hirst, Arwen Hunter, Melanie Kardel, Wing Chang
Life of this patent
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Abstract
The present disclosure relates to improved supplements, culture media and methods for enhancing the survival or proliferation of mammalian stem cells. In particular, adding a lipid supplement, such as a lipid-enriched carrier (e.g. a lipid-enriched albumin), to the culture medium may enhance the survival and/or proliferation of the stem cells by at least 5% to 65% as compared to a culture medium that does not contain the lipid supplement.
Claims12
A cloning supplement comprising one or more small molecule inhibitors and two or more lipids in the presence of a carrier, wherein
- the carrier is an agent, substance, composition or complex that transports some or all of the two or more lipids to a mammalian pluripotent stem cell,
- the two or more lipids comprise oleic acid and palmitic acid,
- the amount of each alpha-linolenic acid and/or arachidonic acid is less than 300 μg per gram of carrier, and
- wherein the survival or proliferation of mammalian pluripotent stem cells is enhanced in a culture medium containing the supplement as compared to a culture medium that does not contain the supplement.
1 more element
Dependencies are inferred from the claim text.