Camp receptor protein variant and method of producing L-amino acid using the same
AssigneeCj Cheiljedang Corporation
InventorsSeok Myung Lee, Ki Yong Cheong, Chang Il Seo, Ji Sun Lee
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Abstract
The present disclosure relates to a variant of cAMP receptor protein of Escherichia coli with alanine at position 35, a microorganism including the same, and a method of producing an L-amino acid using the same.
Claims10
A cAMP receptor protein variant, wherein the cAMP receptor protein variant has transcription regulator activity of a cAMP receptor protein, has at least 80% sequence identity to the sequence of SEQ ID NO:
- 1, and has alanine at the position corresponding to position 35 of SEQ ID NO:
- 1.
A polynucleotide encoding a cAMP receptor protein variant, wherein the cAMP receptor protein variant has transcription regulator activity of a cAMP receptor protein, has at least 80% sequence identity to the sequence of SEQ ID NO:
- 1, and has alanine at the position corresponding to position 35 of SEQ ID NO:
- 1.
A microorganism of the genus Escherichia comprising a cAMP receptor protein variant, wherein the cAMP receptor protein variant has transcription regulator activity of a cAMP receptor protein, has at least 80% sequence identity to the sequence of SEQ ID NO:
- 1, and has alanine at the position corresponding to position 35 of SEQ ID NO:
- 1.
A method of producing an L-amino acid, the method comprising:
- culturing a microorganism of the genus Escherichia in a medium to produce the L-amino acid, wherein the microorganism comprises a cAMP receptor protein variant, wherein the cAMP receptor protein variant has transcription regulator activity of a cAMP receptor protein, has at least 80% sequence identity to the sequence of SEQ ID NO:
- 1, and has alanine at the position corresponding to position 35 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.