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US 11,697,670 B2Granted
Methods for purifying antibodies having reduced high molecular weight aggregates
AssigneeBiogen MA Inc.
InventorsMatthew Westoby, Greg Evangelist
Life of this patent
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Abstract
Provided herein, in some embodiments, are methods and compositions for purifying antibodies from cellular cultures using one or more thiol containing additives during a purification process, for example in a chromatographic purification process.
Claims13
A method of reducing formation of high molecular weight antibody aggregates in an antibody sample, the method comprising:
- subjecting an antibody sample to viral inactivation at a pH of less than 5.0;
- neutralizing the antibody sample to produce a neutralized antibody sample having a pH of greater than 5.0;
- and
- introducing an additive into the neutralized antibody sample, wherein the additive is selected from the group consisting of cysteine, L-cysteine, sulfur dioxide, hydrogen sulfide, bisulfite, and glutathione.
1 more element
A method of reducing formation of high molecular weight antibody aggregates in an antibody sample, the method comprising:
- subjecting an antibody sample to viral inactivation at a pH of less than 5.0, wherein the antibody sample comprises a non-human antibody;
- neutralizing the antibody sample to produce a neutralized antibody sample having a pH of greater than 5.0;
- and
- introducing an additive into the neutralized antibody sample, wherein the additive is selected from the group consisting of cysteine, L-cysteine, sulfur dioxide, hydrogen sulfide, bisulfite, and glutathione.
1 more element
Dependencies are inferred from the claim text.