Methods and devices for de novo oligonucleic acid assembly

A method for polynucleotide synthesis, comprising:

• a) providing predetermined sequences;
• b) providing a device for polynucleotide synthesis, the device comprising:
• i) a structure having a surface, wherein the structure comprises silicon dioxide;
• ii) a plurality of recesses or posts on the surface, wherein each recess or post comprises:
• 1) a width length that is 6.8 nm to 500 nm,
• 2) a pitch length that is about twice the width length, and
• 3) a depth length that is about 60% to about 125% of the pitch length;
• iii) a plurality of loci on the surface, wherein each locus has a diameter of 0.5 to 100 μm, wherein each locus comprises at least two of the plurality of recesses or posts;
• and
• iv) a plurality of clusters on the surface, wherein each of the clusters comprise 50 to 500 loci and has a cross-section of 0.5 to 2 mm
• c) synthesizing a plurality of non-identical polynucleotides at least 10 bases in length, wherein each of the non-identical polynucleotides extends from a different locus, wherein an aggregate deletion error rate is achieved without correcting errors.