Method for preparing phospholipid micelles

A controllable method for preparing phospholipid micelles, comprising:

• S1, preparing small phospholipid vesicles:
• removing solvent of phospholipid chloroform solution, then fully drying the phospholipid chloroform solution in a vacuum drying oven to completely remove chloroform solvents, adding with deionized water and subjecting to ultrasonic treatment under heating;
• then preparing small phospholipid vesicle solution with a size of 100 nano-meters (nm) to 200 nm by extruding with a micro extruder;
• wherein the phospholipid in the S1 is a dipalmitoyl phosphatidic acid or dipalmitoyl phosphatidylcholine;
• the phospholipid in the S1 is in a mass-volume ratio of (1 gram (g) to 2 g):(1 milliliter (mL) to 2 mL) to the deionized water;
• S2, preparing a graphene thin-layer electrode substrate:
• pressing and sticking a transparent adhesive tape on a surface of a flat layer of a large graphene, and quickly tearing down the tape in parallel to obtain a flat graphene thin layer on the transparent adhesive tape, the graphene thin-layer electrode substrate;
• S3, dropwise adding small phospholipid vesicle solution with the size of 100 nm-200 nm and salt solution onto the graphene thin-layer electrode substrate in turn, incubating the substrate at 50-70 degree Celsius (° C.), and washing the substrate to obtain an incubated thin-layer electrode with phospholipid monolayer;
• and
• S4, electroforming phospholipid micelles:
• connecting the incubated thin layer electrode with phospholipid monolayer and a platinum wire electrode to electrodes of an electrochemical workstation respectively under a temperature of 50° C.-70° C. and electrochemical potential of −2 Volts (V)-+2 V, followed by reaction for 2 minutes (min)-10 min to obtain phospholipid micelles with different depths and widths on the incubated thin-layer electrode with phospholipid monolayer;
• maintaining the obtained phospholipid micelles under a stable micellar morphology below 40° C.