Gene expression system for probiotic microorganisms
AssigneeZBiotics Company
InventorZachary Abbott
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Abstract
Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.
Claims20
A composition formulated for oral administration to humans, the composition comprising:
- a recombinant bacterium, wherein the recombinant bacterium constitutively expresses an expression product encoded by a heterologous nucleic acid sequence operatively linked with a flagellin gene promoter,
- wherein the expression product is an aldehyde dehydrogenase (ALDH) enzyme that oxidizes acetaldehyde to acetate,
- wherein the recombinant bacterium is present in the composition in an amount sufficient that, when the composition is orally administered to a human, it metabolizes acetaldehyde in the presence of alcohol.
Dependencies are inferred from the claim text.