Equalizer-based intensity correction for base calling
AssigneeIllumina, Inc.
InventorsEric Jon Ojard, Rami Mehio, Gavin Derek Parnaby, Nitin Udpa, John S. Vieceli
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Abstract
The technology disclosed relates to equalizer-based intensity correction for base calling. In particular, the technology disclosed relates to accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters, selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio, applying the pixel coefficients to intensity values of the pixels in the image to produce an output, and base calling the target cluster based on the output.
Claims26
A computer-implemented method of base calling, the computer-implemented method including:
- accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
- selecting, from a bank of lookup tables, a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
- applying the pixel coefficients to intensity values of the pixels in the image to produce an output;
- and
- base calling the target cluster based on the output.
A non-transitory computer readable storage medium impressed with computer program instructions to perform base calling, the computer program instructions, when executed on a processor, implement a method comprising:
- accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
- selecting, from a bank of lookup tables, a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
- applying the pixel coefficients to intensity values of the pixels in the image to produce an output;
- and
- base calling the target cluster based on the output.
A system including one or more processors coupled to memory, the memory loaded with computer instructions to perform base calling, the computer instructions, when executed on the one or more processors, implement actions comprising:
- accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
- selecting from a bank of lookup tables a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
- applying the pixel coefficients to intensity values of the pixels in the image to produce an output;
- and
- base calling the target cluster based on the output.
A system including one or more processors coupled to memory, the memory loaded with computer instructions to perform base calling, the computer instructions, when executed on the one or more processors, implement actions comprising:
- accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
- selecting, from a bank of lookup tables, a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
- convolving the pixel coefficients with intensity values of the pixels in the image to produce convolved features;
- interpolating the convolved features to generate an output;
- and
- base calling the target cluster based on the output.
Dependencies are inferred from the claim text.