Systems and methods for electrochemical point-of-care detection of hemoglobin

A method for electrochemically detecting free hemoglobin in a sample solution comprising:

• oxidizing the free hemoglobin;
• cleaving fructosyl-valine-histidine (FVH) from the free hemoglobin to form an electrochemical test solution comprising a mixture of hemoglobin, FVH, and hemoglobin degradation products;
• reacting a first portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product;
• measuring a first electrical property of the first reaction product which is indicative of an amount of hemoglobin A1C in the sample solution;
• reacting a second portion of the electrochemical test solution with a chemistry including fructosyl peptide oxidase and ferrocyanide to form a second reaction product;
• measuring a second electrical property of the second reaction product which is indicative of an amount of total hemoglobin in the sample solution;
• and
• determining the amounts of hemoglobin A1C and of total hemoglobin of the sample solution based on the measured first and second electrical properties, respectively.