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US 11,693,006 B2Granted
Blood profiling with protease inhibitors
AssigneeSangui Bio Pty Ltd
InventorsBenjamin Ross Herbert, Elisabeth Karsten
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Abstract
The present disclosure relates to methods for generating blood protein profiles from whole blood, red blood cell enriched blood samples, or red blood cell components. The methods involve the comparison of protein levels before and after incubation with protease inhibitors, or the comparison of protein levels after incubation in the presence and absence of protease inhibitors. The protein profiles are used for monitoring and diagnosing diseases and disorders in subjects including colorectal cancer and preeclampsia.
Claims21
A method of producing a protein profile comprising:
- A)
- a) obtaining a red blood cell component from a blood sample;
- b) obtaining a first and second portion from the red blood cell component;
- c) contacting the second portion from the red blood cell component with one or more protease inhibitors;
- d) measuring the level of one or more proteins from the first portion of the red blood cell component and from the contacted second portion of the red blood cell component, wherein the one or more proteins are selected from the group consisting of chemokines, cytokines, growth factors, receptors, intracellular signal transmitters, hormones, nuclear transcription factors, neurotransmitters, extracellular matrix components, glycoproteins, inflammatory proteins, and enzymes;
- and
- e) determining the difference in the measured level of the one or more proteins from the first portion of the red blood cell component and the contacted second portion of the red blood cell component,
- wherein the protein profile produced comprises the one or more proteins having a difference in the measured level of said one or more proteins from first portion of the red blood cell component and the contacted second portion of the red blood cell component;
- or
- B)
- a) obtaining a red blood cell component from a blood sample;
- b) measuring the level of one or more proteins from the red blood cell component, wherein the one or more proteins are selected from the group consisting of chemokines, cytokines, growth factors, receptors, intracellular signal transmitters, hormones, nuclear transcription factors, neurotransmitters, extracellular matrix components, glycoproteins, inflammatory proteins, and enzymes;
- c) contacting the red blood cell component with one or more protease inhibitors;
- d) measuring the level of the one or more proteins from the contacted red blood cell component;
- and
- e) determining the difference in the measured level of the one or more proteins from the red blood cell component and the contacted red blood cell component;
- wherein the protein profile produced comprises the one or more proteins having a difference in the measured level of said one or more proteins from the red blood cell component and the contacted red blood cell component.
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Dependencies are inferred from the claim text.