Seal enhancer
AssigneeSophion Bioscience A/S
InventorsLars Damgaard Løjkner, Anders Lindqvist, Daniel Rafael Sauter, Kadla Røskva Rosholm
Life of this patent
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Abstract
A seal enhancer for improving the patch clamp seal in a patch clamp method or apparatus is provided. The internal solution comprises particular anions and the external solution comprises one or more metal ions at low concentration.
Claims20
A patch clamp system comprising a patch clamp device, an internal solution and an external solution,
- wherein the internal solution comprises one or more anions selected from phosphate (PO43−) ions, sulfate (SO42−) ions and fluoride (F−) ions;
- wherein the external solution comprises one or more metal ions selected from Ca2+, Sr2+, Ba2+, or combinations thereof;
- wherein a concentration of the one or more metal ions in the external solution is between 1 and 20 mmol/L;
- wherein the internal solution additionally comprises calcium (Ca2+) ions;
- and
- wherein the internal solution has a concentration of fluoride (F−) ions which is less than 5 mmol/L.
A method of making a seal enhancer in a patch clamp system, the method comprising combining one or more anions selected from phosphate (PO43−), sulfate (SO42−) and fluoride (F−) anions with one or more metal ions selected from Ca2+, Sr2+ and Ba2+, or combinations thereof, wherein the anions are present in an internal solution, and the metal ions are present in an external solution of the patch clamp system, wherein the concentration of the one or more metal ions in the external solution is between 1 and 20 mmol/L, wherein the internal solution additionally comprises calcium (Ca2+) ions, and wherein the internal solution has a concentration of fluoride (F−) ions which is less than 5 mmol/L.
A method for providing a gigaseal in a patch clamp system, the patch clamp system comprising a patch clamp device, an internal solution and an external solution, the method comprising the steps of:
- a. introducing a solution of one or more anions selected from phosphate (PO43−—), sulfate (SO42−) and fluoride (F−) anions into the internal solution,
- wherein the internal solution additionally comprises calcium (Ca2+) ions and wherein the internal solution has a concentration of fluoride (F−) ions which is less than 5 mmol/L;
- b. introducing a solution of cells into the external solution, and capturing the cells within the patch clamp device;
- c. introducing a solution of one or more metal ions selected from Ca2+, Sr2+, and Ba2+, or combinations thereof into the external solution;
- wherein a concentration of the one or more metal ions in the external solution is between 1 and 20 mmol/L,
- wherein steps a. and b. can be performed in any order.
A kit for a patch clamp system, the kit comprising an internal solution and an external solution,
- wherein the internal solution comprises one or more anions selected from selected from phosphate (PO43−—), sulfate (SO42−) and fluoride (F−) anions;
- wherein the internal solution additionally comprises calcium (Ca2+) ions;
- wherein the internal solution has a concentration of fluoride (F−) ions which is less than 5 mmol/L;
- wherein the external solution comprises a solution of one or more metal ions selected from Ca2+, Sr2+, and Ba2+, or combinations thereof;
- and
- wherein the one or more metal ions are present in the external solution at a concentration between 1 and 20 mmol/L.
Dependencies are inferred from the claim text.