VOC markers in saliva for diagnosis of gastric cancer and gastric cancer diagnostic method using same
AssigneeShanghai Jiao Tong University
InventorsDaxiang Cui, Cuili Xue, Yunsheng Chen
Life of this patent
Estimating the term…
Abstract
Disclosed herein are a group of gastric cancer VOC markers in saliva and an application thereof in the preparation of a diagnostic reagent of gastric cancer. The markers are a combination of compounds selected from the group consisting of acetaldehyde, 2-methylbutyraldehyde, isopropanol, hexanal, n-butanol, cineole, nonanal, menthone, 2-ethylhexanol, menthol, anethole and dodecanol. The diagnostic reagent is used for detecting the contents of the marker in a saliva sample of a subject to perform the diagnosis of gastric cancer.
Claims2
A method of diagnosing gastric cancer in a subject, the method comprising steps of:
- a) obtaining a saliva sample from the subject;
- b) detecting volatile organic compound (VOC) markers in the saliva sample by:
- concentrating the VOC markers in the saliva sample using a solid-phase microextraction device;
- and
- performing an analysis using a gas chromatography-mass spectrometry under a temperature program of 35° C. for 10 minutes (min), rise to 200° C. at a rate of 8° C./min, rise to 220° C. at a rate of 15° C./min and 220° C. for 12 min,
- wherein the VOC markers are compounds selected from the group consisting of acetaldehyde, isopropanol, hexanal, n-butanol, nonanal, menthone, menthol, anethole, dodecanol, and a combination thereof;
- and
- c) diagnosing the subject with gastric cancer when at least three of the compounds of acetaldehyde, isopropanol, hexanal, n-butanol and nonanal are present and at least three of the compounds of menthone, menthol, anethole and dodecanol are absent in the saliva sample.
A method of diagnosing gastric cancer in a subject, the method comprising steps of:
- a) obtaining a saliva sample from the subject;
- b) detecting volatile organic compound (VOC) markers in the saliva sample by:
- concentrating the VOC markers in the saliva sample using a solid-phase microextraction device;
- and
- performing an analysis using a gas chromatography-mass spectrometry under a temperature program of 35° C. for 10 min, rise to 200° C. at a rate of 8° C./min, rise to 220° C. at a rate of 15° C./min and 220° C. for 12 min,
- wherein the VOC markers are compounds selected from the group consisting of acetaldehyde, isopropanol, hexanal, n-butanol, nonanal, menthone, menthol, anethole, dodecanol, and a combination thereof;
- c) preliminarily diagnosing the subject with gastric cancer when significant increase in content of 2-methylbutyraldehyde, cineole and 2-ethylhexanol is detected;
- and
- d) diagnosing the subject with gastric cancer when at least three of the compounds of acetaldehyde, isopropanol, hexanal, n-butanol and nonanal are present and at least three of the compounds of menthone, menthol, anethole and dodecanol are absent in the saliva sample.
Dependencies are inferred from the claim text.