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US 11,692,964 B2Granted
Methods and apparatus for measuring analytes
AssigneeLife Technologies Corporation
InventorsMark James Milgrew, Jonathan M. Rothberg, James Bustillo
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Abstract
Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
Claims15
A method for operating a sensor array, comprising:
- creating a correction factor data set for the sensor array before initiating an analysis using the sensor array, wherein creating a correction factor data set includes:
- calculating a first transition value data set from a first reference data set acquired at a first data rate that is less than or equal to a reciprocal of a settling time;
- calculating a second transition value data set from a second reference data set acquired at a second data rate that is greater than the reciprocal of the settling time;
- calculating a correction factor for each sensor of the sensor array from the first transition value data set and the second transition value data set;
- and
- storing each correction factor as the correction factor data set;
- acquiring an analysis data set from the array at the second data rate during the analysis;
- and
- applying the correction factor data set to the analysis data set.
6 more elements
Dependencies are inferred from the claim text.