A method of inspection for detecting defects on a substrate for a reflective reticle, wherein the substrate is configured to be illuminated by radiation having an actinic wavelength to expose an object with a pattern of the reticle, the method comprising performing the inspection using inspection radiation obtained from a high harmonic generation source, the inspection radiation having one or more wavelengths within a wavelength range of between 20 nm and 150 nm and the one or more wavelengths being approximately an integer multiple, other than 1, of the actinic wavelength.