Spectral edge detection
AssigneeRareCyte, Inc.
InventorJeremy Ryan Cooper
Life of this patent
Estimating the term…
Abstract
This disclosure relates generally to detecting multiple biomarkers on or within a sample, though more specifically, to detecting individual detection moieties within a plurality of detection moieties.
Claims32
A method of analyzing a sample, the sample comprising a first component, the first component having a first autofluorescent emission spectrum, the first autofluorescent emission spectrum having a peak emission intensity at a peak intensity wavelength, a leading spectral edge with emission intensities less than the peak intensity at wavelengths less than the peak intensity wavelength, and a trailing spectral edge with emission intensities less than the peak intensity at wavelengths greater than the peak intensity wavelength, the method comprising:
- acquiring, by stimulating the sample with excitation light, a first raw image of the sample at a first emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the first emission wavelength is in the leading spectral edge or in the trailing spectral edge of the first autofluorescent emission spectrum, and wherein the first raw image comprises a first total signal comprising a first autofluorescent signal at the first emission wavelength;
- acquiring, by stimulating the sample with excitation light, a second raw image of the sample at a second emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the second raw image comprises a second total signal comprising a second autofluorescent signal at the second emission wavelength;
- and
- outputting a final image of the first component based on the first and second raw images, and
- wherein the first component comprises a non-detection moiety.
A non-transitory computer readable medium comprising instructions to cause one or more processors or devices to perform a method of analyzing a sample, the sample comprising a first component, the first component having a first autofluorescent emission spectrum, the first autofluorescent emission spectrum having a peak emission intensity at a peak intensity wavelength, a leading spectral edge with emission intensities less than the peak intensity at wavelengths less than the peak intensity wavelength, and a trailing spectral edge with emission intensities less than the peak intensity at wavelengths greater than the peak intensity wavelength, the method comprising:
- acquiring, by stimulating the sample with excitation light, a first raw image of the sample at a first emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the first emission wavelength is in the leading spectral edge or in the trailing spectral edge of the first autofluorescent emission spectrum, and wherein the first raw image comprises a first total signal comprising a first autofluorescent signal at the first emission wavelength;
- acquiring, by stimulating the sample with excitation light, a second raw image of the sample at a second emission wavelength of the first autofluorescent emission spectrum of the first component, wherein the second emission wavelength, and wherein the second raw image comprises a second total signal comprising a second autofluorescent signal at the second emission wavelength;
- and
- outputting a final image of the first component based on the first and second raw images, and
- wherein the first component comprises a non-detection moiety.
Dependencies are inferred from the claim text.