Spectral calibration apparatus and spectral calibration method

A spectral calibration apparatus for calculating a color conversion matrix used in color conversion processing, comprising:

• a spectral signal acquisition unit that acquires a spectral signal of fluorescence detected over time;
• an initial calculation unit the calculates initial values of a color conversion matrix and initial values of a temporal activation of fluorescence of the spectral signal by
• removing a spike signal from the spectral signal, to obtain a spike-less signal,
• removing a baseline from the spike-less signal, to obtain a modified signal,
• normalizing an intensity between wavelength channels based on the modified signal, to obtain a normalized signal,
• calculating the initial values of the color conversion matrix using the normalized signal, and
• calculating the initial values of the temporal activation of fluorescence using the initial values of the color conversion matrix;
• a candidate calculation unit that calculates a candidate of the color conversion matrix for each value of a parameter using the initial values of the color conversion matrix and the initial values of the temporal activation of fluorescence, which depends on a frequency at which fluorescence peaks of fluorescent dyes appear at the same time, based on the spectral signal;
• and
• a selection unit that selects a color conversion matrix based on an evaluation value calculated for each candidate.