Animal model of non-alcoholic liver disease and composition of diagnosis, prevention or treatment for non-alcoholic liver disease
AssigneeKorea Advanced Institute of Science and Technology
InventorsDae-Sik Lim, Sun Hye Jeong
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Abstract
The present invention provides a method of manufacturing an animal model of non-alcoholic liver disease by using correlation among metabolic dysregulations through AKT regulation by Hippo signaling, and an animal model prepared by the method above, and a screening method of a therapeutic agent by using the animal model.
Claims9
An animal model of non-alcoholic liver disease in which the Pten and Sav1 genes are deleted specifically in a liver, wherein the non-alcoholic liver disease is selected from the group consisting of non-alcoholic fatty liver, non-alcoholic hepatosteatitis, cirrhosis, and liver cancer.
A method of manufacturing an animal model for non-alcoholic liver disease in which the Pten and Sav1 genes are deleted specifically in a liver, comprising:
- obtaining a first-generation animal by mating an animal of Ptenf/f genotype and an animal of Albumin-Cre genotype expressing CRE;
- obtaining a second-generation animal by mating the first-generation animal and an animal of Sav1f/f genotype;
- and
- obtaining a third-generation animal comprising Ptenf/f;Sav1f/f;Albumin-Cre genotype by mating between the second-generation animals.
Dependencies are inferred from the claim text.