Construction of next generation sequencing (NGS) libraries using competitive strand displacement

A sequencing adapter comprising:

• a first DNA strand and a second DNA strand;
• wherein the first DNA strand comprises a nucleotide sequence of SEQ ID NO:
• 18 that includes a 5′ phosphate and a blocking group at its 3′ end;
• wherein the second DNA strand comprises a nucleotide sequence of SEQ ID NO:
• 34 that includes a dideoxy nucleotide (ddN) at its 3′ end;
• and
• wherein the second DNA strand is at least partially complementary to the first DNA strand.