US 11,692,210 B2Granted

Anaerobic fermentative production of furandicarboxylic acid

AssigneeBraskem S.A.

InventorsIuri Estrada Gouvea, Marcos Rogerio Simoes, Mariana Trovo Marchesin, Aline Silva Romão Dumaresq

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Abstract

The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.

Claims23

Dependent claims

A recombinant microorganism that is an ethanol-producing yeast comprising:

(a) at least one exogenous nucleic acid molecule encoding a polypeptide that catalyzes the production of 4-hydroxymethylfurfural phosphate from glyceraldehyde 3-phosphate (G3P);
(b) at least one endogenous and/or exogenous nucleic acid molecule encoding a polypeptide that catalyzes the production of 4-hydroxymethylfurfural (4-HMF) from 4-hydroxymethylfurfural phosphate;
(c) at least one endogenous and/or exogenous nucleic acid molecule encoding one or more polypeptides that catalyze the production of 2,4-furandicarboxylic acid (2,4-FDCA) from 4-HMF through the production of the intermediates furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 4-formylfuran-2-carboxylate, 4-formylfuran-2-carboxylate, and/or 2-formylfuran-4-carboxylate, wherein the production of 2,4-FDCA leads to the reduction of (i) NAD to NADH or (ii) NADP to NADPH;
(d) at least one nucleic acid molecule encoding a polypeptide that catalyzes the production of ribulose-1,5-bisphosphate from ribulose-5-phosphate;
and
(e) at least one nucleic acid molecule encoding a polypeptide that catalyzes the production of two molecules of glycerate-3-phosphate from the combination of CO2 with ribulose-1,5-bisphosphate;
and
wherein the recombinant microorganism utilizes the glycerate-3-phosphate from (e) and the NADH and/or NADPH generated as a byproduct of 2,4-FDCA production at (c) to produce ethanol;
and
wherein the 2,4-FDCA and ethanol are coproduced under anaerobic or microaerobic conditions.

7 more elements

A recombinant microorganism that is an ethanol-producing yeast comprising:

(a) at least one exogenous nucleic acid molecule encoding a polypeptide that catalyzes the production of 4-hydroxymethylfurfural phosphate from glyceraldehyde 3-phosphate (G3P);
(b) at least one endogenous and/or exogenous nucleic acid molecule encoding a polypeptide that catalyzes the production of 4-hydroxymethylfurfural (4-HMF) from 4-hydroxymethylfurfural phosphate;
(c) at least one endogenous and/or exogenous nucleic acid molecule encoding one or more polypeptides that catalyze the production of 2,4-furandicarboxylic acid (2,4-FDCA) from 4-HMF through the production of the intermediates furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 4-formylfuran-2-carboxylate, 4-formylfuran-2-carboxylate, and/or 2-formylfuran-4-carboxylate, wherein the production of 2,4-FDCA leads to the reduction of (i) NAD to NADH or (ii) NADP to NADPH;
(d) at least one nucleic acid molecule encoding a polypeptide that catalyzes the production of acetyl phosphate and D-glyceraldehyde-3-phosphate from D-xylulose-5-phosphate and phosphate, and/or at least one nucleic acid molecule encoding a polypeptide that catalyzes the production of acetyl phosphate and erythrose-4-phosphate from fructose-6-phosphate and phosphate;
(e) at least one nucleic acid molecule encoding one or more polypeptides that catalyze:
(1) the production of acetyl-CoA from acetyl phosphate and free coenzyme A;
and/or
(2) the production of acetate from acetyl phosphate and the production of acetyl-CoA from acetate;
and
(f) at least one nucleic acid molecule encoding one or more polypeptides that catalyze:
(1) the production of acetaldehyde from acetyl-CoA;
and/or
(2) the production of ethanol from acetyl-CoA;
and;
wherein the recombinant microorganism utilizes the NADH and/or NADPH generated as a byproduct of 2,4-FDCA production at (c) to produce ethanol, and;
wherein the 2,4-FDCA and ethanol are coproduced under anaerobic or microaerobic conditions.

13 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/464,139Filed 1 Sep 2021 · us provisional application
Priority: 1 Sep 2020
Published: US 2022/0064683 A13 Mar 2022
Granted: 4 Jul 2023
Term ends: Not estimatedThe source records don’t support an estimate.

Classification31 codes

C12P 17/04
Oxygen as only ring hetero atoms: containing a five-membered hetero ring, e.g. griseofulvin , vitamin C
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 9/0008
Oxidoreductases: acting on the aldehyde or oxo group of donors
C12N 9/1029
Acyltransferases: transferring groups other than amino-acyl groups