Systems and methods for one-shot guide RNA (ogRNA) targeting of endogenous and source DNA
AssigneeEditas Medicine, Inc.
InventorsAri E. Friedland, Hariharan Jayaram, Barrett Ethan Steinberg
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Abstract
Engineered nucleic acids encoding genome editing system components are provided, as are engineered RNA-guided nucleases that include inserts encoded in part by cellular genomic or other sequences recognized by guide RNAs.
Claims6
An isolated nucleic acid encoding an RNA-guided nuclease and comprising a eukaryotic nucleic acid sequence, wherein the eukaryotic nucleic acid sequence is at least 17 nucleotides in length and either comprises or is adjacent to a protospacer adjacent motif (PAM) that is recognized by the RNA-guided nuclease, wherein the RNA-guided nuclease is a Cas9 protein, and wherein the nucleic acid encoding the Cas9 protein comprises a sequence having at least 95% sequence identity to SEQ ID NO:
- 1 and comprising an insertion of c.157insN19-36.
Dependencies are inferred from the claim text.