US 11,692,200 B2 · Method for improving rice yield and/or rice blast resistance and protein used thereof

Abstract

The invention discloses a method for improving rice yield and a protein used thereof. The invention provides a method for cultivating the target rice, comprising the following steps of inhibiting the activity of RAY1 protein in original rice to obtain target rice; compared with the original rice, the target rice shows higher yield and/or larger grain size and/or stronger resistance to rice blast and/or higher plant height and/or longer stem internode length; the RAY1 protein is a protein composed of an amino acid sequence shown as SEQ ID No. 1 in a sequence list. The invention uses CRISPR/Cas9 technology to realize site-directed editing rice RAY1 gene, through knocking out rice RAY1 gene by frameshift mutation, the protein RAY1 is inactivated, and a new generation of rice germplasm with significantly improved yield is obtained.

Claims5

Dependent claims

A method for producing a rice variety, the method comprising:

transforming a sgRNA expression vector into an original rice variety using a CRISPR/Cas9 method to produce a target rice variety,
wherein the sgRNA expression vector comprises a target sequence as set forth in SEQ ID NO:
8,
wherein the transformation causes a loss of function in a gene encoding the RAY1 protein of SEQ ID NO:1;
wherein said gene has the polynucleotide sequence of SEQ ID NO:2 or SEQ ID NO:3;
and
wherein the target rice variety exhibits an increase in an attribute selected from the group consisting of yield, grain size, resistance to rice blast, height, and internode lengthening relative to the original rice variety.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/427,570Filed 21 Jan 2020

Priority

CN 201910107261.62 Feb 2019

Published

US 2022/0106605 A17 Apr 2022

Granted

4 Jul 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 15/113
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: non-coding nucleic acids modulating the…
C12N 15/8213
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…

Method for improving rice yield and/or rice blast resistance and protein used thereof

Life of this patent

Method for improving rice yield and/or rice blast resistance and protein used thereof

Life of this patent

Abstract

Claims5