Back to results
US 11,692,198 B2Granted
Targeted gene activation in plants
AssigneeThe Regents of the University of California
InventorsSteve E. Jacobsen, Ashot Papikian
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to the targeting of transcriptional activators to specific loci in plants to activate transcription of the targeted loci. Specifically, the present disclosure provides methods and compositions for using guided (e.g. RNA-guided) transcriptional activators to activate transcription of specific loci in plants.
Claims19
A method for activating expression of a target nucleic acid in a plant, comprising:
- (a) providing a plant comprising:
- a first recombinant polypeptide comprising 1) a nuclease-deficient CAS9 polypeptide (dCAS9) or fragment thereof and 2) a multimerized epitope;
- a second recombinant polypeptide comprising 1) a histone methyltransferase domain at least 90% identical to SEQ ID NO:
- 98 and 2) an affinity polypeptide that specifically binds to the epitope;
- a crRNA and a tracrRNA, or fusions thereof;
- and
- (b) growing the plant under conditions whereby the first and second recombinant polypeptides are targeted to the target nucleic acid, thereby activating expression of the target nucleic acid.
4 more elements
A recombinant vector comprising:
- a first nucleic acid sequence comprising a plant promoter and that encodes a recombinant polypeptide comprising 1) a nuclease-deficient CAS9 polypeptide (dCAS9) or fragment thereof and 2) a multimerized epitope;
- a second nucleic acid sequence comprising a plant promoter and that encodes a recombinant polypeptide comprising 1) a histone methyltransferase domain at least 90% identical to SEQ ID NO:
- 98 and 2) an affinity polypeptide that specifically binds to the epitope;
- and
- a third nucleic acid sequence comprising a promoter and that encodes a crRNA and a tracrRNA, or fusions thereof.
2 more elements
A method for producing a plant with increased expression of a target nucleic acid, comprising:
- (a) providing a plant comprising a recombinant nucleic acid, wherein the recombinant nucleic acid encodes a recombinant polypeptide comprising:
- 1) histone methyltransferase domain at least 90% identical to SEQ ID NO:
- 98, and 2) a heterologous DNA-binding domain comprising a zinc finger domain and capable of binding a target nucleic acid;
- and
- (b) growing the plant under conditions whereby the recombinant polypeptide encoded by the recombinant nucleic acid is expressed and is targeted to the target nucleic acid, thereby increasing expression of the target nucleic acid to produce the plant with increased expression of the target nucleic acid.
2 more elements
Dependencies are inferred from the claim text.