Delivery of biological molecules to plant cells
AssigneeInari Agriculture Technology, Inc.
InventorsJohn Paul Issa, Grethel Yanet Busot
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Abstract
Methods and systems for delivering biological materials such as nucleic acids and/or proteins to the interior of a plant cell are provided. Such methods and systems include those where biological material is non-covalently complexed with a magnetic particle and accelerated multiple times toward a plant cell.
Claims22
A method of delivering a biological material to the interior of a plant cell, comprising accelerating a biological material/magnetic particle complex multiple times toward a plant cell, wherein the biological material is non-covalently complexed with the magnetic particle, wherein the plant cell is a soybean plant cell that has an intact cell wall, wherein the biological material comprises a material selected from the group consisting of a polynucleotide, a polypeptide, a ribonucleoprotein, a sequence-specific genome editing agent, and a combination thereof and wherein the biological material/magnetic particle complex is accelerated toward the plant cell multiple times by consecutive application of a static magnetic field to the plant cell and biological material/magnetic particle complex followed by separation of the static magnetic field from the plant cell and the biological material/magnetic particle complex, such that the static magnetic field is applied and then discontinued repeatedly, whereby the biological material is delivered to the interior of the plant cell.
A method of providing a genome editing agent to a soybean plant cell, comprising multiple applications of a static magnetic field by consecutive application of the magnetic field followed by withdrawal of the magnetic field to a composition including (a) a plant tissue containing a soybean plant cell, wherein the plant cell has an intact cell wall;
- and (b) magnetic nanoparticles of about 150 to about 300 nanometers in diameter that are non-covalently complexed with a genome editing agent;
- whereby the genome editing agent is delivered to the interior of the plant cell.
A plant modification system comprising:
- (a) a sequence-specific nuclease, or a polynucleotide encoding a sequence-specific nuclease, or a ribonucleoprotein including at least one sequence-specific nuclease non-covalently complexed with an approximately spherical magnetic nanoparticle of about 150 to about 300 nanometers in diameter;
- (b) a pollen grain comprising a soybean plant cell;
- (c) a medium containing exogenous non-specific DNA;
- and (d) a magnet that provides a static magnetic field that is applied and then discontinued repeatedly.
Dependencies are inferred from the claim text.