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US 11,692,194 B2Granted
Modulating expression of polypeptides via new gene switch expression systems
AssigneePrecigen, Inc.
InventorsRutul R. Shah, Thomas D. Reed, Cheryl G. Bolinger
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Abstract
Disclosed herein are polynucleotides encoding ligand-inducible gene switch polypeptides, and systems comprising gene switch polypeptides for modulating the expression of a heterologous gene and an interleukin in a host cell. The compositions, methods and systems described herein facilitate ligand dependent expression of polypeptides including but not limited to cytokines and antigen binding polypeptides.
Claims32
A vector comprising a polynucleotide encoding:
- (a) a first gene switch polypeptide comprising a transactivation domain fused to a first ligand binding domain;
- and (b) a second gene switch polypeptide comprising a DNA binding domain fused to a second ligand binding domain, wherein:
- the first and second gene switch polypeptides are connected by a polypeptide linker;
- and
- the first gene switch polypeptide comprises an amino acid sequence having at least 90% identity with SEQ ID NO:
- 183 and/or the second gene switch polypeptide comprises an amino acid sequence having at least 90% identity with SEQ ID NO:
- 187 or 188.
4 more elements
Dependencies are inferred from the claim text.