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US 11,692,189 B2Granted
Methods for purification of messenger RNA
AssigneeTranslate Bio, Inc.
InventorsFrank DeRosa, Anusha Dias, Michael Heartlein, Shrirang Karve
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Abstract
The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow filtration, wherein the mRNA purified from step (b) is substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis.
Claims14
A method of manufacturing messenger RNA (mRNA) comprising:
- (a) synthesizing mRNA in vitro to provide an impure preparation of mRNA;
- and
- (b) purifying the mRNA,
- wherein purifying the mRNA comprises:
- (i) subjecting the impure preparation of mRNA to a denaturing condition;
- and
- (ii) subjecting the treated impure preparation from step (b)(i) to tangential flow filtration,
- thereby manufacturing mRNA.
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Dependencies are inferred from the claim text.