Angiogenin expression in plants
AssigneeAgriculture Victoria Services PTY LTD
InventorsGerman Spangenberg, Aidyn Mouradov, Jianghui Wang, Benjamin Graeme Cocks, Matthew Knight, Matthew McDonagh
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Abstract
The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.
Claims10
A plant cell, wherein the plant cell is part of a monocotyledon or a dicotyledon plant callus, plant, seed or other plant part, said plant cell containing a nucleic acid encoding an angiogenin, wherein said nucleic acid encoding an angiogenin comprises:
- (a) an N-terminal plant signal sequence that when expressed as the N-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component selected from mitochondria, chloroplast, nucleus and nucleolus, and
- (b) a nucleotide sequence encoding a functional angiogenin protein, optimized for expression in a monocotyledon or a dicotyledon plant cell said monocotyledon optimized nucleotide sequence selected from the group consisting of:
- (i) SEQ ID NO:
- 22, and
- (ii) variants of SEQ ID NO:
- 22 having at least 98% sequence identity to the full length of SEQ ID NO:
- 22;
- and
- said dicotyledon optimized nucleotide sequence selected from the group consisting of:
- (iii) SEQ ID NO:
- 23, and
- (iv) variants of SEQ ID NO:
- 23, having at least 98% sequence identity to the full length of SEQ ID NO:
- 23,
- wherein said nucleic acid provides for a production of angiogenin protein at a level of between approximately 5 and 30% (weight/volume) of the total soluble protein of said plant cell,
- wherein said monocotyledon is selected from the group consisting of forage grasses, sorghum, sugarcane, corn, oat, wheat, rice, and barley, and
- wherein said dicotyledon is selected from the group consisting of forage legumes, soybean, lupin, peas, lentils, chickpeas, canola, vegetable brassicas, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed, and safflower.
An artificial construct comprising a nucleic acid encoding an angiogenin and a promoter, operatively linked to the nucleic acid encoding an angiogenin,
- wherein the promoter is effective for enabling expression of the nucleic acid encoding an angiogenin in a monocotyledon or dicotyledon plant cell;
- and
- wherein said nucleic acid encoding an angiogenin comprises
- (a) an N-terminal plant signal sequence that when expressed as the N-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component selected from mitochondria, chloroplast, nucleus and nucleolus, and
- (b) a nucleotide sequence encoding a functional angiogenin protein optimized for expression in a monocotyledon or a dicotyledon plant cell said monocotyledon optimized nucleotide sequence selected from the group consisting of:
- (i) SEQ ID NO:
- 22, and
- (ii) variants of SEQ ID NO:
- 22 having at least 98% sequence identity to the full length of SEQ ID NO:
- 22;
- and
- said dicotyledon optimized nucleotide sequence selected from the group consisting of:
- (iii) SEQ ID NO:
- 23, and
- (iv) variants of SEQ ID NO:
- 23, having at least 98% sequence identity to the full length of SEQ ID NO:
- 23,
- wherein said nucleic acid provides for a production of angiogenin protein at a level of between approximately 5% and 30% (weight/volume) of the total soluble protein of a transfected plant cell,
- wherein said monocotyledon is selected from the group consisting of forage grasses, sorghum, sugarcane, corn, oat, wheat, rice, and barley, and
- wherein said dicotyledon is selected from the group consisting of forage legumes, soybean, lupin, peas, lentils, chickpeas, canola, vegetable brassicas, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed, and safflower.
Dependencies are inferred from the claim text.