Angiogenin expression in plants
AssigneeAgriculture Victoria Services PTY LTD
InventorsGerman Spangenberg, Aidyn Mouradov, Jianghui Wang, Benjamin Graeme Cocks, Matthew Knight, Matthew McDonagh
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Abstract
The present invention relates to plant-produced angiogenins, to related plant cells, plant calli, plants, seeds and other plant parts and products derived therefrom and to uses of plant-produced angiogenins. The present invention also relates to expression of angiogenin genes in plants and to related nucleic acids, constructs and methods.
Claims10
A plant cell, wherein the plant cell is part of a, plant, seed or other plant part, said plant cell containing a nucleic acid encoding an angiogenin, wherein said nucleic acid encoding an angiogenin comprises
- (a) a C-terminal plant signal peptide that when expressed as the C-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component, and
- (b) a nucleotide sequence encoding a functional angiogenin protein selected from the group consisting of:
- (i) SEQ ID NO:
- 52, and
- (ii) variants of SEQ ID NO:
- 52, having at least 98% sequence identity to the full length of SEQ ID NO:
- 52, and
- wherein said plant is selected from the group consisting of a grass, a forage legume, soybean, lupin, peas, lentils, chickpeas, vegetable brassica, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed and safflower.
An artificial construct comprising a nucleic acid encoding an angiogenin and a promoter, operatively linked to the nucleic acid encoding an angiogenin, wherein the promoter is effective for enabling expression of the nucleic acid encoding an angiogenin in a plant cell;
- and
- wherein said nucleic acid encoding an angiogenin comprises
- (a) a C-terminal plant signal sequence that when expressed as the C-terminus of an angiogenin protein drives angiogenin accumulation in the plant cell to a sub-cellular component, and
- (b) a nucleotide sequence encoding a functional angiogenin protein selected from the group consisting of:
- (i) SEQ ID NO:
- 52, and
- (ii) variants of SEQ ID NO:
- 52, having at least 98% sequence identity to the full length of SEQ ID NO:
- 52, and
- wherein said plant cell is located in a plant selected from the group consisting of a grass, a forage legume, soybean, lupin, peas, lentils, chickpeas, vegetable brassica, lettuce, spinach, bananas, citrus, strawberries, apples, oil palm, linseed, cottonseed and safflower.
Dependencies are inferred from the claim text.