RNA-guided DNA nucleases and uses thereof
AssigneeMonsanto Technology LLC
InventorsJaishree Chittoor, Ervin Nagy, Keith H. Turner
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Abstract
Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.
Claims32
A non-naturally occurring system for sequence-specific modification of a target nucleic acid sequence, the system comprising:
- (a) one or more guide RNAs or a DNA molecule encoding the one or more guide RNAs;
- and (b) a CRISPR enzyme having the amino acid sequence of SEQ ID NO:73 or polynucleotide encoding the CRISPR enzyme, wherein the one or more guide RNAs and the CRISPR enzyme do not naturally occur together.
A recombinant nucleic acid, comprising a heterologous promoter operably linked to a polynucleotide encoding a CRISPR enzyme having the amino acid sequence of SEQ ID NO:
- 73.
Dependencies are inferred from the claim text.