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US 11,692,175 B2Granted
Methods for generation of pluripotent and multipotent cells
AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsDavid D. Roberts, Sukhbir Kaur, Jeffrey S. Isenberg
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Abstract
This disclosure relates to methods of producing induced pluripotent (iPS), multipotent, and/or lineage-committed stem cells from differentiated cells, maintaining iPS, multipotent, and/or lineage-committed cells in culture, and re-differentiating the iPS and multipotent stem cells into any desired lineage-committed cell type.
Claims21
A method for inducing multipotent or lineage-committed stem cells, comprising:
- obtaining primary cells from an animal;
- culturing the primary cells;
- and
- contacting the cultured primary cells with an agent that blocks CD47 signaling, thereby inducing multipotent or lineage-committed stem cells.
1 more element
A method for generating differentiated cells, comprising:
- obtaining primary cells from an animal;
- culturing the primary cells;
- contacting the cultured primary cells with an agent that blocks CD47 signaling to produce contacted cells;
- isolating from the contacted cells, multipotent or lineage committed stem cells that express at least one of c-Myc, Sox2, Klf4, and Oct4;
- and
- culturing the isolated multipotent or lineage-committed stem cells in cell differentiation medium to produce differentiated cells.
3 more elements
Dependencies are inferred from the claim text.