Preparation method of human-derived cardiac stem cell spheroid and use thereof
AssigneesInje University Industry-Academic Cooperation Foundation, Hierabio Inc.
InventorsSeung Jin Lee, Young-il Yang
Life of this patent
Estimating the term…
Abstract
The present invention relates to a preparation method of a spheroid using human-derived cardiac stem cells and a therapeutic use for ischemic heart disease using the myocardial regeneration effect thereof. The spheroid using the cardiac stem cells provided in the present invention has excellent myocardial differentiation ability and regenerative therapeutic ability as compared to existing cardiac stem cells, and thus may be used for the treatment of ischemic heart disease such as myocardial infarction.
Claims9
A method for preparing a cardiac stem cell spheroid, comprising,
- culturing the cardiac stem cells (CSCs) within 3 days in a culture vessel that inhibits cell adhesion,
- wherein the culture vessel comprises a medium comprising serum at a concentration of 0.1% to 1%, and
- wherein the CSCs are seeded at a density of between 1×102 cells/cm2 to 2×105 cells/cm2 in the medium.
Dependencies are inferred from the claim text.