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US 11,692,167 B2Granted
Chemically defined serum replacements for cell culture
AssigneeMontana State University
InventorElizabeth Dale Corbin
Life of this patent
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Abstract
Disclosures herein are directed to chemically defined animal-derived component free supplements designed for individual cell types that supports the ex vivo growth of cells as well or better than serum, in chemically defined conditions.
Claims18
A chemically defined media supplement comprising:
- (a) Ascorbyl palmitate, docosahexanoic acid, palmitoleic acid, cholesterol sulfate and at least two components selected from the group consisting of ascorbic acid, silver, vitamin A, vitamin D3, and vitamin K;
- or
- (b) ITSE (Insulin, transferrin, selenium, ethanolamine), Trace Elements A, Alpha Linolenic Acid, fibroblast growth factor, insulin-like growth factor, oleic acid, docosahexanoic acid, and vitamin D3;
- or
- (c) ITSE (Insulin, transferrin, selenium, ethanolamine), Trace Elements A, Alpha Linolenic Acid, fibroblast growth factor, insulin-like growth factor, cholesterol sulfate and palmitoleic acid;
- wherein the chemically defined media supplement is animal derived product free.
3 more elements
Dependencies are inferred from the claim text.