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US 11,692,160 B2Granted
Microfluidic chip, three-dimensional channel structure, cell culture method using same, and activity evaluation method of bioactive substance using same
AssigneesThe Asan Foundation, University of Ulsan Foundation for Industry Cooperation
InventorsGi Seok Jeong, Changmo Hwang, Se-Jin Jang
Life of this patent
Estimating the term…
Abstract
The microfluidic chip according to an embodiment of the present invention may include a plate, a bridge channel formed in intaglio on one side of the plate, an inlet formed through the plate to communicate with one end of the bridge channel, an outlet formed through the plate to communicate with the other end of the bridge channel, and at least one well extending in an outward direction of the plate from the bridge channel to provide a space, wherein the bridge channel may be in the form of a curved line, a bent line, an arc, a circle, a spiral, or a polygon.
Claims14
A microfluidic chip comprising:
- a plate;
- a bridge channel formed in intaglio on one side of the plate;
- an inlet formed through the plate to communicate with one end of the bridge channel;
- an outlet formed through the plate to communicate with another end of the bridge channel;
- and
- a plurality of wells extending in an outward direction of the plate from the bridge channel to provide a space,
- wherein a portion between said one end of the bridge channel and said the other end of the bridge channel has a circular shape, and
- wherein the plurality of wells extend radially outward from the circular shaped portion of the bridge channel and are disposed opposite side of a center of the circular shaped portion with respect to the bridge channel, and each of the plurality of wells has a largest width at a radial inner edge thereof so that different types of cells injected into the microfluidic chip are placed in the plurality of wells when a centrifugal force is generated by a rotation of the microfluidic chip.
5 more elements
A microfluidic chip comprising:
- a plate;
- a plurality of bridge channels formed in intaglio on one side of the plate;
- a plurality of inlets formed by penetrating through the plate, the inlets respectively communicating with one ends of the plurality of bridge channels;
- a plurality of outlets formed by penetrating through the plate, the outlets respectively communicating with other ends of the bridge channels;
- and
- a plurality of wells extending in an outward direction of the plate from each of the plurality of bridge channels to provide a space,
- wherein a portion between said one end of each of the bridge channels and said the other end of each of the bridge channels has a circular shape, and
- wherein the plurality of wells extend radially outward from the circular shaped portion of each of the bridge channels and are disposed opposite side of a center of the circular shaped portion with respect to each of the bridge channels, and each of the plurality of wells has a largest width at a radial inner edge thereof so that different types of cells injected into the microfluidic chip are placed in the plurality of wells when a centrifugal force is generated by a rotation of the microfluidic chip.
5 more elements
A method for culturing cells, comprising:
- injecting cells into a microfluidic chip;
- rotating the microfluidic chip;
- and
- culturing the cells in the microfluidic chip,
- wherein the microfluidic chip is provided with an inlet, an outlet, a bridge channel connecting the inlet and the outlet, and at least one well formed in an outward direction of the bridge channel, and the cells are cultured in the well,
- wherein the microfluidic chip includes:
- a plate;
- a bridge channel formed in intaglio on one side of the plate;
- an inlet formed through the plate to communicate with one end of the bridge channel;
- an outlet formed through the plate to communicate with another end of the bridge channel;
- and
- a plurality of wells extending in an outward direction of the plate from the bridge channel to provide a space,
- wherein a portion between said one end of the bridge channel and said the other end of the bridge channel has a circular shape, and
- wherein the plurality of wells extend radially outward from the circular shaped portion of the bridge channel and are disposed opposite side of a center of the circular shaped portion with respect to the bridge channel, and each of the plurality of wells has a largest width at a radial inner edge thereof so that different types of cells injected into the microfluidic chip are placed in the plurality of wells when a centrifugal force is generated by a rotation of the microfluidic chip.
11 more elements
A method for evaluating an activity of a physiologically active substance, comprising:
- injecting cells into a microfluidic chip;
- rotating the microfluidic chip to supply the cells to a well of the microfluidic chip;
- injecting a biomimetic cell support into the microfluidic chip;
- and
- injecting a physiologically active candidate substance into the microfluidic chip,
- wherein the microfluidic chip is provided with an inlet, an outlet, a bridge channel connecting the inlet and the outlet, and at least one well formed in an outward direction of the bridge channel,
- wherein the microfluidic chip includes:
- a plate;
- a bridge channel formed in intaglio on one side of the plate;
- an inlet formed through the plate to communicate with one end of the bridge channel;
- an outlet formed through the plate to communicate with another end of the bridge channel;
- and
- a plurality of wells extending in an outward direction of the plate from the bridge channel to provide a space,
- wherein a portion between said one end of the bridge channel and said the other end of the bridge channel has a circular shape, and
- wherein the plurality of wells extend radially outward from the circular shaped portion of the bridge channel and are disposed opposite side of a center of the circular shaped portion with respect to the bridge channel, and each of the plurality of wells has a largest width at a radial inner edge thereof so that different types of cells injected into the microfluidic chip are placed in the plurality of wells when a centrifugal force is generated by a rotation of the microfluidic chip.
12 more elements
A method for preparing an organoid, comprising:
- injecting cells into a microfluidic chip;
- rotating the microfluidic chip;
- and
- culturing the cells in the microfluidic chip,
- wherein the microfluidic chip is provided with an inlet, an outlet, a bridge channel connecting the inlet and the outlet, and at least one well formed in an outward direction of the bridge channel,
- wherein the cells include at least one selected from tumor cells, organ cells, stem cells, or a combination thereof, and
- wherein the cells are cultured in the well,
- wherein the microfluidic chip includes:
- a plate;
- a bridge channel formed in intaglio on one side of the plate;
- an inlet formed through the plate to communicate with one end of the bridge channel;
- an outlet formed through the plate to communicate with another end of the bridge channel;
- and
- a plurality of wells extending in an outward direction of the plate from the bridge channel to provide a space,
- wherein a portion between said one end of the bridge channel and said the other end of the bridge channel has a circular shape, and
- wherein the plurality of wells extend radially outward from the circular shaped portion of the bridge channel and are disposed opposite side of a center of the circular shaped portion with respect to the bridge channel, and each of the plurality of wells has a largest width at a radial inner edge thereof so that different types of cells injected into the microfluidic chip are placed in the plurality of wells when a centrifugal force is generated by a rotation of the microfluidic chip.
13 more elements
Dependencies are inferred from the claim text.