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AssigneeAldevron, LLC
InventorIsabelle Louise Chambers
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The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.
A method of growing bacterial host cells in a gas-permeable bag comprising the steps of:
A method of producing plasmid DNA from bacterial host cells grown in a gas-permeable bag comprising the steps of:
A method of expressing a protein in bacterial host cells grown in a gas-permeable bag comprising the steps of:
Dependencies are inferred from the claim text.