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US 11,692,025 B2Granted
Single domain binding molecule
AssigneeElasmogen Ltd
InventorsCaroline Barelle, Mischa Roland Muller, Valerie Calabro, Jack Bikker, John Steven, Lioudmila Tchistiakova, Oleg Kovalenko, Andrea Olland
Life of this patent
Estimating the term…
Abstract
The present invention provides a single domain specific binding molecule having the structure FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4 in which the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, the Complementarity Determining Regions CDR1 and CDR3, and the Hypervariable Regions HV2, and HV4 have amino acid sequences as defined which provide a high affinity anti-human serum albumin (HSA) binding domain.
Claims34
A fusion protein comprising:
- A) a single domain specific binding molecule comprising the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, in which:
- i) FW1 comprises TRVDQSPSSLSASVGDRVTITCVLT (SEQ ID NO:
- 28), ARVDQSPSSLSASVGDRVTITCVLR (amino acids 1-25 of SEQ ID NO:
- 67) or TRVDQTPRTATRETGESLTINCVLT (SEQ ID NO:
- 1),
- ii) FW2 comprises TYWYQQKPGS (SEQ ID NO:
- 29), TCWYQQKPGK (amino acids 34-43 of SEQ ID NO:
- 67), or TYWYRKNPGS (SEQ ID NO:
- 2),
- iii) FW3a comprises GRYSESVN (SEQ ID NO:
- 30), or GRYVESVN (SEQ ID NO:
- 3),
- iv) FW3b comprises FTLTISSLQPEDFATYYCRA (SEQ ID NO:
- 31), FTLTISSLQPEDFATYYCGL (amino acids 65-84 of SEQ ID NO:
- 67) or FSLRIKDLTVADSATYICRA (SEQ ID NO:
- 4), and
- v) FW4 comprises GAGTKVEIK (SEQ ID NO:
- 32), CGQGTKVEIK (amino acids 103-112 of SEQ ID NO:
- 67) or GAGTVLTVN (SEQ ID NO:
- 5);
- or
- B) a single domain specific binding molecule comprising framework regions FW1, FW2, FW3a, FW3b, and FW4 in which:
- i) FW1 comprises a sequence having at least 50% identity to TRVDQSPSSLSASVGDRVTITCVLT (SEQ ID NO:
- 28), ARVDQSPSSLSASVGDRVTITCVLR (amino acids 1-25 of SEQ ID NO:
- 67) or TRVDQTPRTATRETGESLTINCVLT (SEQ ID NO:
- 1),
- ii) FW2 comprises a sequence having at least 50% identity to TYWYQQKPGS (SEQ ID NO:
- 29), TCWYQQKPGK (amino acids 34-43 of SEQ ID NO:
- 67, or TYWYRKNPGS (SEQ ID NO:
- 2),
- iii) FW3a comprises a sequence having at least 50% identity to GRYSESVN (SEQ ID NO:
- 30), or GRYVESVN (SEQ ID NO:
- 3),
- iv) FW3b comprises a sequence having at least 50% identity to FTLTISSLQPEDFATYYCRA (SEQ ID NO:
- 31), FTLTISSLQPEDFATYYCGL (amino acids 65-84 of SEQ ID NO:
- 67) or FSLRIKDLTVADSATYICRA (SEQ ID NO:
- 4), and
- v) FW4 comprises a sequence having at least 50% identity to GAGTKVEIK (SEQ ID NO:
- 32), CGQGTKVEIK (amino acids 103-112 of SEQ ID NO:
- 67) or GAGTVLTVN (SEQ ID NO:
- 5).
38 more elements
Dependencies are inferred from the claim text.