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US 11,692,020 B2Granted
Cytokine fusion proteins, and their pharmaceutical compositions and therapeutic applications
AssigneeAnwita Biosciences, Inc.
InventorsZiyang Zhong, Fan Ye, Matthew Siegel, Jianing Huang
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Abstract
Provided herein are a fusion protein comprising first and second cytokine domains, and a half-life extension domain; and a pharmaceutical composition thereof. Also provided herein are methods of their use for treating, preventing, or ameliorating one or more symptoms of a proliferative disease.
Claims34
A fusion protein comprising an interleukin-2 domain, an interleukin-21 domain, and an albumin binding domain;
- wherein the interleukin-2 domain comprises the amino acid sequence of SEQ ID NO:
- 1, 2, 3, 4, or 5;
- or wherein the interleukin-2 domain is an N-glycosylated interleukin-2 comprising one, two, three, or four substitutions at position P34, K35, L36, T37, R38, M39, L40, T41, F42, K43, F44, Y45, M46, P47, E61, E62, L63, K64, P65, L66, E67, E68, V69, L70, N71, L72, A73, Q74, Y107, D109, and/or T111 as set forth in the amino acid sequence of SEQ ID NO:
- 1, 2, 3, 4, or 5;
- wherein the interleukin-21 domain comprises the amino acid sequence of SEQ ID NO:
- 156, 157, or 158;
- and
- wherein the C-terminus of the interleukin-2 domain is connected to the N-terminus of the interleukin-21 domain directly or via a peptide linker;
- and the C-terminus of the interleukin-21 domain is connected to the N-terminus of the albumin binding domain directly or via a peptide linker;
- or
- wherein the C-terminus of the interleukin-21 domain is connected to the N-terminus of the interleukin-2 domain directly or via a peptide linker;
- and the C-terminus of the interleukin-2 domain is connected to the N-terminus of the albumin binding domain directly or via a peptide linker;
- or
- wherein the C-terminus of the interleukin-2 domain is connected to the N-terminus of the albumin binding domain directly or via a peptide linker;
- and the C-terminus of the albumin binding domain is connected to the N-terminus of the interleukin-21 domain directly or via a peptide linker;
- or
- wherein the C-terminus of the interleukin-21 domain is connected to the N-terminus of the albumin binding domain directly or via a peptide linker;
- and the C-terminus of the albumin binding domain is connected to the N-terminus of the interleukin-2 domain directly or via a peptide linker;
- or
- wherein the C-terminus of the albumin binding domain is connected to the N-terminus of the interleukin-2 domain directly or via a peptide linker;
- and the C-terminus of the interleukin-2 domain is connected to the N-terminus of the interleukin-21 domain directly or via a peptide linker;
- or
- wherein the C-terminus of the albumin binding domain is connected to the N-terminus of the interleukin-21 domain directly or via a peptide linker;
- and the C-terminus of the interleukin-21 domain is connected to the N-terminus of the interleukin-2 domain directly or via a peptide linker.
21 more elements
Dependencies are inferred from the claim text.