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US 11,692,015 B2Granted
Modified fibroin
AssigneesSpiber Inc., Riken
InventorsKeisuke Morita, Yunosuke Abe, Takehisa Maekawa, Koichi Kotaka, Junichi Sugahara, Keiji Numata
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Abstract
The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)nmotif-REP]m or Formula 2: [(A)nmotif-REP]m−(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin.
Claims23
A modified fibroin, comprising:
- a domain sequence represented by Formula 1:
- [(A)n motif-REP]m or Formula 2:
- [(A)n motif-REP]m−(A)n motif, wherein the domain sequence has an amino acid sequence equivalent with a reduced content of a glutamine residue to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin wherein
- in Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more,
- REP represents an amino acid sequence consisting of 10 to 200 amino acid residues and contains a GPGXX (where X represents an amino acid residue other than a glycine residue) motif and has a GPGXX motif content of 10% or more, wherein an increase in the GPGXX motif content increases tensile strength and elasticity,
- m represents an integer of 10 to 300,
- a plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences, and
- a plurality of REPs may be the same amino acid sequence or different amino acid sequences.
5 more elements
A modified fibroin, comprising:
- a domain sequence represented by Formula 1:
- [(A)n motif-REP]m or Formula 2:
- [(A)n motif-REP]m−(A)n motif,
- wherein a glutamine residue content rate is 9% or less,
- wherein a hydrophobicity of the REP is −0.8 or more,
- wherein in Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 10 to 300, a plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences, and a plurality of REPs may be the same amino acid sequence or different amino acid sequences, wherein an increase in the (A)n motif content increases tensile strength and elasticity.
3 more elements
A modified fibroin, comprising:
- a domain sequence represented by Formula 1:
- [(A)n motif-REP]m or Formula 2:
- [(A)n motif-REP]m−(A)n motif,
- wherein a glutamine residue content rate is 9% or less,
- wherein the REP contains a GPGXX (where X represents an amino acid residue other than a glycine residue) motif and has a GPGXX motif content of 10% or more, and an increase in the GPGXX motif content increases tensile strength and elasticity,
- wherein in Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 10 to 300, a plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences, and a plurality of REPs may be the same amino acid sequence or different amino acid sequences.
3 more elements
Dependencies are inferred from the claim text.