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AssigneeNitto Denko Corporation
InventorsMasafumi Iwamoto, Tsuyoshi Mukobata, Akira Yokouchi
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This invention is intended to discover a novel solvent that can be used as an alternative to toluene in the step of deprotection in the method of solid-phase nucleic acid synthesis. With the use of such novel solvent, various problems caused by the use of toluene are dissolved. This invention is also intended to provide a method of solid-phase nucleic acid synthesis in which protected nucleoside phosphoramidites in which a protective group is bonded to a hydroxyl group at the 5′position or the 3′ position of a nucleoside are sequentially bound on a solid phase carrier, where a reaction of removing the protecting group from the protected nucleoside phosphoramidite is carried out in a solution comprising an acid with a pKa of 0.2 to 0.8 and acetonitrile.
A method of solid-phase nucleic acid synthesis in which protected nucleoside phosphoramidites in which a protective group is bonded to a hydroxyl group at the 5′ position or the 3′ position of a nucleoside are sequentially bound on a solid phase carrier, comprising deprotection of the hydroxyl group by removing the protecting group from the protected nucleoside phosphoramidite in a solution comprising an acid with a pKa of 0.2 to 0.8 and acetonitrile.
Dependencies are inferred from the claim text.