US 11,692,002 B2 · RNA sequence adaptation

Abstract

The present invention is directed to a method for modifying the retention time of RNA on a chromatographic column. The present invention also concerns a method for purifying RNA from a mixture of at least two RNA species. Furthermore, the present invention relates to a method for co-purifying at least two RNA species from a mixture of at least two RNA species. In particular, the present invention provides a method for harmonizing the numbers of A and/or U nucleotides in at least two RNA species. The present invention is also directed to RNA obtainable by said methods, a composition comprising said RNA or a vaccine comprising said RNA and methods for producing such RNA and compositions. Further, the invention concerns a kit, particularly a kit of parts, comprising the RNA, composition or vaccine. The invention is further directed to a method of treating or preventing a disorder or a disease, first and second medical uses of the RNA, composition and vaccine. Moreover, the present invention concerns a method for providing an adapted RNA sequence or an adapted RNA mixture.

Claims22

Dependent claims

A method for analysis or purification of a mixture comprising at least two harmonized RNA species, the method comprising:

a) obtaining the coding sequences for at least two RNA species, said at least two RNA species each having a length of 800 to 20,000 nucleotides wherein the sequence of at least one RNA species is adapted by altering the number of A and/or U nucleotides in the RNA sequence with respect to the number of A and/or U nucleotides in the original RNA sequence, said coding sequences of the at least two RNA species having a harmonized number of encoded A and U nucleotides that is no more than 50 different from each other;
b) synthesizing the at least two RNA species to produce at least two harmonized RNA species;
and
c) analysing and/or purifying a mixture of said at least two harmonized RNA species by chromatography.

1 more element

Dependencies are inferred from the claim text.

RNA sequence adaptation

Life of this patent

RNA sequence adaptation

Life of this patent

Abstract

Claims22