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US 11,690,921 B2Granted
Delivery of target specific nucleases
AssigneesSangamo Therapeutics, Inc., Life Technologies Corporation
InventorsAnthony Conway, Xavier de Mollerat du Jeu, Shikha Mishra
Life of this patent
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Abstract
Described herein are lipid nanoparticles comprising cationic lipids and other lipids and also comprising engineered nucleases facilitate transfer of nucleic acids to cells.
Claims24
A cationic lipid nanoparticle (LNP) comprising one or more polynucleotides encoding one or more zinc finger protein (ZFP) transcription factors (ZFP-TFs) or one or more zinc finger nucleases (ZFNs), wherein the ZFP-TFs or ZFNs comprise a ZFP designated 56691 or 56690, wherein
- (i) the ZFP designated 56691 comprises 6 zinc finger domains ordered F1 to F6, the zinc finger domains comprising the following recognition helix regions:
- F1:
- DRSHLTR (SEQ ID NO:
- 6);
- F2:
- QSGDLTR (SEQ ID NO:
- 7);
- F3:
- DRSNRTT (SEQ ID NO:
- 8);
- F4:
- RSDALAR (SEQ ID NO:
- 9);
- F5:
- RSDNLSE (SEQ ID NO:
- 10);
- and
- F6:
- ERANRNS (SEQ ID NO:
- 11);
- and
- (ii) the ZFN designated 56690 comprises 6 zinc finger domains ordered F1 to F6, the zinc finger domains comprising the following recognition helix regions:
- F1:
- DRSALSR (SEQ ID NO:
- 12);
- F2:
- TSGSLTR (SEQ ID NO:
- 13);
- F3:
- QSSDLSR (SEQ ID NO:
- 14);
- F4:
- WRKSLKV (SEQ ID NO:
- 15);
- F5:
- DRSHLTR (SEQ ID NO:
- 6);
- and
- F6:
- RLDWLPM (SEQ ID NO:
- 16).
38 more elements
Dependencies are inferred from the claim text.