General and direct method for preparing oligonucleotide-functionalized metal-organic framework nanoparticles
AssigneeNorthwestern University
InventorsChad A. Mirkin, Shunzhi Wang
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Abstract
The present disclosure generally relates to metal-organic framework nanoparticles containing terminal phosphate-modified oligonucleotides, methods for making the same, and methods of using the same.
Claims34
An oligonucleotide-functionalized metal-organic framework (MOF) nanoparticle, wherein the oligonucleotide is a terminal phosphate-modified oligonucleotide and the phosphate forms a metal-phosphate bond with the metal ion of the MOF nanoparticle, wherein the terminal phosphate-modified oligonucleotide comprises a (GGT)n nucleotide sequence, wherein n is 2-20.
A method of making an oligonucleotide-functionalized metal-organic framework (MOF) nanoparticle, comprising:
- (a) mixing a metal ion and a multi-dentate ligand to form the MOF nanoparticle;
- and
- (b) contacting the MOF nanoparticle with a plurality of terminal phosphate-modified oligonucleotides, wherein one or more of the plurality of terminal phosphate-modified oligonucleotides comprises a (GGT)n nucleotide sequence, wherein n is 2-20, thereby producing the oligonucleotide-functionalized MOF nanoparticle, such that the phosphate groups of the terminal phosphate-modified oligonucleotides associate with coordinatively unsaturated metal sites (CUS) on the MOF nanoparticle surface via a metal-phosphate bond.
Dependencies are inferred from the claim text.