MHC class I associated peptides for prevention and treatment of multiple flavi virus
AssigneeEmergex Vaccines Holding Ltd.
InventorRamila Philip
Life of this patent
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Abstract
The invention provides a vaccine composition comprising a flavi peptide comprising one or more CD8+ T cell epitopes.
Claims20
A vaccine composition comprising a flavivirus peptide comprising one or more of the CD8+T cell epitopes set out in SEQ ID NOs:
- 1 to 14, 16, 17 and 19 to 23 or a variant thereof,
- wherein the flavivirus peptide is 8 to 30 amino acids in length;
- wherein said flavivirus peptide is attached to a nanoparticle;
- and
- wherein said variants differ from any one of SEQ ID NOs 1 to 14, 16, 17 or 19 to 23 by no more than one amino acid deletion or insertion, or no more than one conservative amino acid substitution.
A vaccine composition comprising a polynucleotide encoding a flavivirus peptide comprising one or more of the CD8+T cell epitopes set out in SEQ ID NOs:
- 1 to 14, 16, 17 and 19 to 23 or a variant thereof;
- wherein the flavivirus peptide is 8 to 30 amino acids in length,
- and wherein said variants differ from any one of SEQ ID NOs 1 to 14, 16, 17 or 19 to 23 by no more than one amino acid deletion or insertion, or no more than one conservative amino acid substitution,
- and wherein said polynucleotide encoding the flavivirus peptide is attached to a nanoparticle.
A method for generating cytotoxic T lymphocytes (CTLs) for use in passive immunotherapy, comprising contacting T cells obtained from a subject infected with a flavivirus with a flavivirus peptide, wherein the flavivirus peptide is 8 to 30 amino acids in length and comprises one or more of the CD8+T cell epitopes set out in SEQ ID NOs:
- 1 to 14, 16, 17 and 19 to 23 or a variant thereof;
- wherein said variants differ from any one of SEQ ID NOs 1 to 14, 16, 17 or 19 to 23 by no more than one amino acid deletion or insertion, or no more than one conservative amino acid substitution;
- and, optionally wherein the CTLs are autologous to or HLA-matched with a recipient of the CTLs.
A method for diagnosing a flavivirus infection in a subject, comprising (i) contacting T cells obtained from the subject with a flavivirus peptide, wherein the flavivirus peptide is 8 to 30 amino acids in length and comprises one or more of the CD8+T cell epitopes set out in SEQ ID NOs:
- 1 to 14, 16, 17 and 19 to 23 or a variant thereof;
- wherein said variants differ from any one of SEQ ID NOs 1 to 14, 16, 17 or 19 to 23 by no more than one amino acid deletion or insertion, or no more than one conservative amino acid substitution;
- and (ii) determining the response of the T cells to the flavivirus peptide.
Dependencies are inferred from the claim text.