HU specific interfering agents
AssigneeResearch Institute At Nationwide Children's Hospital
InventorsLauren O. Bakaletz, Steven D. Goodman
Life of this patent
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Abstract
Methods of and compositions for breaking down a biofilm or inhibiting, preventing or treating a microbial infection that produces a biofilm are disclosed, which involves administration of an interfering agent capable of specifically competing, titrating, or inhibiting the binding of an HU protein to a microbial DNA. By competing with HU proteins that bind to DNA scaffold in the biofilm, these interfering agents destabilize the biofilm leading to destruction and removal of the biofilm by the immune system. Further method and composition aspects are contemplated in relation to infections caused by bacteria that export an HU protein.
Claims15
A composition comprising a glycosidic polymer-based microsphere, wherein the glycosidic polymer has a diameter of 20 μm to 50 μm and comprises an insoluble cross-linked dextran and an effective amount of an isolated antibody or an antigen-binding fragment thereof that specifically recognizes or binds to a histone-like (HU) polypeptide of Streptococcus gordonii consisting of the amino acid sequence of SEQ ID NO:
- 12 and an effective amount of an isolated antibody or an antigen-binding fragment thereof that specifically recognizes or binds to a histone-like (HU) polypeptide of Porphyromonas gingivalis consisting of the amino acid sequence of SEQ ID NO:
- 11.
A glycosidic polymer-based microsphere comprising an insoluble cross-linked dextran having a diameter of 20 μm to 50 μm, wherein the microsphere is bound by an isolated antibody or an antigen-binding fragment thereof that specifically recognizes or binds to a histone-like (HU) polypeptide of Streptococcus gordonii consisting of the amino acid sequence of SEQ ID NO:
- 12 and an isolated antibody or an antigen-binding fragment thereof that specifically recognizes or binds to a histone-like (HU) polypeptide of Porphyromonas gingivalis consisting of the amino acid sequence of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.