Umbilical cord-derived adherent stem cells, preparation method therefor, and use thereof

A composition comprising enhanced adherent stem cells that are isolated from a single source and cultured under a hypoxia condition and a pharmaceutically acceptable carrier,

• wherein the single source is a mammalian umbilical cord, and
• wherein the enhanced adherent stem cells have the following characteristics (a) to (f):
• (a) having a high expression level of TAGLN, and having a high expression of one or more selected from the group consisting of STC1, LRRC17, and IL33, as compared with bone marrow stem cells;
• (b) having a low expression level of ANXA4, TPMT, and NAKG, and having a low expression level of one or more selected from the group consisting of CCND1, SERPINE1, and PRNP, as compared with bone marrow stem cells;
• (c) maintaining the morphology of adherent fibroblasts during subculture;
• (d) having ability to differentiate into adipocytes, osteocytes, and chondrocytes;
• (e) having one or more surface antigen characteristics selected from the group consisting of CD200+, Tra1-60−, CD3−, CD1a−, CD11c−, CD16−, CD86a−, CD8a−, CD40−, CD141+, CD61+, CD87+, and MIC A/B−;
• and
• (f) having surface antigen characteristics of Oct4− and Nanog−.