Universal chimeric antigen receptor T cells specific for CD22

A Chimeric Antigen Receptor (CAR) specific for CD22 (Anti-CD22 CAR) comprising:

• i) at least one extracellular domain comprising an antigen binding domain specific for CD22, and a hinge domain comprising an FcRIIIα, a CD8α, an IgG1, an IgG4, or a PD1 hinge;
• ii) a transmembrane domain;
• iii) an intracellular signaling domain comprising a CD3zeta signaling domain comprising an amino acid sequence having at least 95% identity with SEQ ID NO:
• 9;
• and
• iv) at least one monoclonal antibody (mAb)-specific epitope(s) selected from the following sequences:
• CPYSNPSLC (SEQ ID NO:
• 91), NSELLSLINDMPITNDQKKLMSNN (SEQ ID NO:
• 114), CQFDLSTRRLKC (SEQ ID NO:
• 115), CQYNLSSRALKC (SEQ ID NO:
• 116), CVWQRWQKSYVC (SEQ ID NO:
• 117), SFVLNWYRMSPSNQTDKLAAFPEDR (SEQ ID NO:
• 119), SGTYLCGAISLAPKAQIKE (SEQ ID NO:
• 120), ELPTQGTFSNVSTNVSPAKPTTTA (SEQ ID NO:
• 92), and GQNDTSQTSSPS (SEQ ID NO:
• 121);
• wherein said antigen binding domain specific for CD22 comprises a single chain variable fragment (scFv) specific for CD22, said scFv comprising a heavy chain variable (VH) region and a light chain variable (VL) region comprising one of the following combinations of sequences, respectively:
• SEQ ID NO:
• 71 and SEQ ID NO:
• 72;
• SEQ ID NO:
• 73 and SEQ ID NO:
• 74;
• SEQ ID NO:
• 75 and SEQ ID NO:
• 76;
• SEQ ID NO:
• 77 and SEQ ID NO:
• 78;
• SEQ ID NO:
• 79 and SEQ ID NO:
• 80;
• SEQ ID NO:
• 81 and SEQ ID NO:
• 82;
• SEQ ID NO:
• 83 and SEQ ID NO:
• 84;
• SEQ ID NO:
• 85 and SEQ ID NO:
• 86;
• SEQ ID NO:
• 87 and SEQ ID NO:
• 88;
• or SEQ ID NO:
• 89 and SEQ ID NO:
• 90.