Systems and methods for optogenetic imaging
AssigneeInscopix, Inc.
InventorsMark O. Trulson, Alice Stamatakis, Koen Visscher
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Abstract
Provided herein are systems and methods for simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system. The microscope can comprise in part an imaging light source and a stimulation light source. Light from the imaging light source and the stimulation light source can be spectrally separated to reduce cross talk between the stimulation light and the imaging light.
Claims20
A microscope system for simultaneous imaging and stimulation of tissue within a subject, the microscope system comprising:
- a) an optical assembly that includes:
- i. an illumination optical path comprising one or more light-emitting elements and configured to direct imaging light to the tissue within a specified field-of-view;
- ii. a stimulation optical path comprising one or more light-emitting elements and configured to direct stimulation light to the tissue within said field-of-view, to the tissue outside of said field-of-view, or to the tissue partially within said field-of-view;
- iii. an imaging optical path comprising one or more image sensors and configured to receive light reflected, scattered, or emitted by the tissue to generate an image of the tissue within said field-of-view;
- b) a corrective optical element optically cooperated with an element of the optical assembly to achieve a spatial resolution of better than 2 μm over the entirety of the specified field-of-view;
- and
- a baseplate is configured to be mounted on the subject in a fixed position and to receive the optical assembly, thereby aligning the optical assembly relative to the tissue within the subject upon attachment to the baseplate, and wherein the optical assembly is removable from the baseplate.
A microscope system for simultaneous imaging and stimulation of tissue within a subject, the microscope system comprising:
- a) an optical assembly that includes
- i. an illumination optical path comprising one or more light-emitting elements and configured to direct imaging light to the tissue within a specified field-of-view;
- ii. a stimulation optical path comprising one or more light-emitting elements and configured to direct stimulation light to the tissue within said field-of-view, to tissue outside of said field-of-view, or to the tissue partially within said field-of-view;
- iii. an imaging optical path comprising one or more image sensors and configured to receive light reflected, scattered, or emitted by the tissue to generate an image of the tissue within said field-of-view;
- and
- iv. one or more deformable lenses configured to adjust a corresponding focal depth of the imaging optical path,
- the stimulation optical path, or both over a range of about 300 μm without loss of spatial resolution;
- and
- b). a baseplate configured to be mounted on the subject in a fixed position and to receive the optical assembly, thereby aligning the optical assembly relative to the tissue within the subject upon attachment to the baseplate, and wherein the optical assembly is removable from the baseplate.
Dependencies are inferred from the claim text.