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US 11,690,544 B2Granted
Method of producing thin enzyme-based sensing layers on planar sensors
AssigneeCalifornia Institute of Technology
InventorsSamson Chen, Axel Scherer, Dvin Adalian, Peter Petillo, Muhammad Musab Jilani, Xiomara L. Madero, Deepan Kishore Kumar
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Abstract
A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.
Claims14
A sensor, comprising:
- a masking layer deposited onto a substrate comprising a plurality of electrodes, wherein the masking layer covers the plurality of electrodes;
- an adhesion promoter deposited onto the substrate and onto the masking layer, wherein deposition of the adhesion promoter onto the plurality of electrodes is avoided due to the masking layer;
- a signal transduction enzyme spin coated onto the substrate after the masking layer is removed from the substrate being coated with the adhesion promoter,
- wherein the signal transduction enzyme is configured to detect an active compound capable of generating an electric current, and
- wherein the signal transduction enzyme is configured so that a current measured by the plurality of electrodes settles within about 5% of its stable value within one second;
- and
- a crosslinker deposited onto the signal transduction enzyme for crosslinking the signal transduction enzyme to produce a cross-linked enzyme film having a thickness of less than one micrometer.
4 more elements
Dependencies are inferred from the claim text.