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US 11,690,514 B2Granted
Device, system and method for quantifying fluorescence and optical properties
AssigneeUniversity Health Network
InventorsAnthony Taywon Kim, Brian Campbell Wilson
Life of this patent
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Abstract
Methods for quantifying fluorescence and optical properties in a turbid medium such as tissue. Devices and systems suitable for the methods are also disclosed.
Claims28
A method for determining a quantitative concentration of a fluorophore in a target medium, the method comprising:
- detecting fluorescence emission from a target surface of the target turbid medium, the detected fluorescence emission including fluorescence emission of the fluorophore generated using a fluorescence excitation wavelength provided to the target turbid medium, wherein the detected fluorescence emission comprises a detected fluorescence spectrum;
- detecting diffuse reflectance over a spectral range of wavelengths from the target surface at one or more known distances from one or more broadband excitation sources to obtain one or more respective diffuse reflectance spectra;
- determining absorption and transport scattering coefficients of the target turbid medium using the one or more respective diffuse reflectance spectra and a priori knowledge of absorption and transport scattering spectra of a known turbid medium;
- and
- calculating a quantitative fluorescence emission spectrum of the fluorophore by correcting the detected fluorescence spectrum for the effects of light absorption and scattering by the target turbid medium using a fluorescence model based on the determined absorption and transport scattering coefficients of the target turbid medium;
- and
- determining the quantitative concentration of the fluorophore in the target turbid medium based on a magnitude or shape of the quantitative fluorescence emission spectrum.
4 more elements
A system for determining a quantitative concentration of a in a target turbid medium, the system comprising:
- a probe configured to:
- provide a fluorescence excitation wavelength and broadband wavelengths to the target turbid medium;
- detect fluorescence emission from a target surface of the target turbid medium, the detected fluorescence emission including fluorescence emission from the fluorophore and comprising a detected fluorescence spectrum;
- and
- detect the diffuse reflectance over a spectral range of wavelengths from the target surface at one or more known distances from one or more broadband excitation sources to obtain one or more respective diffuse reflectance spectra;
- a spectrometer for measuring the detected fluorescence emission and diffuse reflectance;
- and
- a processing device configured to:
- determine absorption and transport scattering coefficients of the target turbid medium using the one or more diffuse reflectance spectra and a priori knowledge of absorption and transport scattering spectra of a known turbid medium;
- and
- calculate a quantitative fluorescence emission spectrum of the fluorophore by correcting the detected fluorescence spectrum for the effects of light absorption and scattering by the target turbid medium using a fluorescence model based on the determined absorption and transport scattering coefficients of the target turbid medium;
- and
- determine the quantitative concentration of the fluorophore in the target turbid medium based on a magnitude or shape of the quantitative fluorescence emission spectrum.
10 more elements
Dependencies are inferred from the claim text.