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US 11,688,595 B2Granted
Operating a mass spectrometer for sample quantification
OwnerPhilip M. Remes, Michael Maccossinventors
Life of this patent
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Abstract
Targeted quantification for mass spectrometry is described. In one aspect, a mass spectrometer can generate survey mass spectra and identify the compounds of a sample using the survey mass spectra. Compounds that elute within a same time range and do not form interfering product ions upon fragmentation can be identified, and grouped together for an MS2 scan. A series of MS2 scans can then be generated to acquire MS2 mass spectra.
Claims17
A method of operating a mass spectrometer to analyze a sample, comprising:
- receiving a sample;
- performing MS2 scans of the sample to generate survey mass spectra, the MS2 scans performed by advancing a first isolation window across a mass range of interest;
- identifying, using the survey mass spectra, a first compound in a first mass range within the mass range of interest and a second compound in a second mass range within the mass range of interest that elutes to the mass spectrometer within a first time range and form product ions when fragmented that do not interfere with each other within a threshold range;
- fragmenting, based on the identification, the first compound and the second compound isolated using a second isolation window having a mass range that at least partially includes the first mass range and the second mass range to form the product ions;
- acquiring a first product ion mass spectrum based on the product ions fragmented from the first compound and the second compound;
- and
- quantifying the first compound and the second compound based on the first product ion mass spectrum.
4 more elements
A mass spectrometer configured to receive a sample, and having a controller circuit configured to:
- perform product ion scans of the sample to generate survey mass spectra, the product ion scans performed by advancing a first isolation window across a mass range of interest;
- identify, using the survey mass spectra, a first compound in a first mass range within the mass range of interest and a second compound in a second mass range within the mass range of interest that elute to the mass spectrometer within a first time range and form product ions when fragmented that do not interfere with each other within a threshold range;
- fragment the first compound and the second compound isolated using a second isolation window having a mass range that includes at least portions of the first mass range and the second mass range to form the product ions;
- and
- acquire a first product ion mass spectrum based on the product ions fragmented from the first compound and the second compound.
2 more elements
Dependencies are inferred from the claim text.