Method for presymptomatic diagnosis of coeliac disease and gluten sensitivity
AssigneeAeneas GmbH & Co. KG
InventorsTorsten Matthias, Kai Prager, Christian Meesters, Patricia Jeremias, Sascha Pfeiffer
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Abstract
The invention relates to the use of an immunologically reactive microbial transglutaminase or its immunologically reactive parts or analogues, which are present in a complex with gliadin or its immunologically reactive parts or analogues, for the diagnosis and/or therapy control of coeliac disease or sprue as well as gluten sensitivity, and a kit for determining the diagnosis and/or therapy control of coeliac disease or sprue as well as of gluten sensitivity, by means of the previously mentioned complex.
Claims8
An in vitro method for diagnosis of a disease and/or monitoring the treatment of the disease comprising:
- obtaining a body fluid sample from a subject in need of diagnosis and/or monitoring;
- contacting a complex of (a) a microbial transglutaminase and (b) a gliadin or gliadin peptides with the body fluid sample;
- and
- using the complex of microbial transglutaminase and a gliadin or gliadin peptides to identify the presence of antibodies in the body fluid sample which bind to the complex;
- wherein the subject is identified as having the disease when antibodies which bind to the complex in the body fluid sample are identified,
- wherein (a) comprises the amino acid sequence of SEQ ID NO:
- 1, and (b) comprises the amino acid sequence of SEQ ID NO:
- 2,
- wherein the disease is selected from the list consisting of coeliac disease, sprue, and gluten sensitivity;
- and,
- wherein the antibodies which bind to the complex formed from microbial transglutaminase and gliadin or gliadin peptides are detectable even before antibodies produced against a complex of tissue transglutaminase and gliadin are detectable.
Dependencies are inferred from the claim text.