Detection and treatment of neurological diseases
AssigneeCity of Hope
InventorsYanhong Shi, Li Li
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Abstract
Disclosed are methods of detecting abnormal expression of one or more genes associated with a neurological disease such as the Alexander disease, the Alzheimer's disease, the Parkinson disease, the Huntington disease, multiple sclerosis, and amyotrophic lateral sclerosis. The methods include performing a transcriptome analysis of the astrocytes derived from a patient and the astrocytes derived from a healthy control subject, thereby to determine one or more genes that are substantially differentially expressed. Also disclosed are methods of treating a neurological disease by correcting the abnormally expressed genes associated with the neurological disease.
Claims7
A method of determining if abnormal expression of a Chitinase-3-like 1 protein (CHI3L1) gene and/or a CHI3L1 receptor is associated with a neurological disease, comprising:
- differentiating astrocytes from induced pluripotent stem cells (iPSCs) obtained from one or more healthy control subjects;
- differentiating astrocytes from iPSCs obtained from a subject suffering from the neurological disease;
- performing a transcriptome analysis of the astrocytes derived from the subject suffering from the neurological disease and a transcriptome analysis of the astrocytes derived from the one or more healthy control subjects;
- comparing the results of both transcriptome analyses to determine if the CHI3L1 gene and/or the CHI3L1 receptor are substantially differentially expressed in the subject suffering from the neurological disease compared to the one or more healthy subjects;
- and if the CHI3L1 gene and/or a CHI3L1 receptor are differentially expressed;
- and
- correcting the expression of the CHI3L1 gene and/or the CHI3L1 receptor,
- wherein complete or partial restoration of one or more phenotypes of the neurological disease after correcting expression of the CHI3L1 gene and/or the CHI3L1 receptor indicates that the CHI3L1 gene and/or the CHI3L1 receptor are associated with the neurological disease.
Dependencies are inferred from the claim text.